Enzyme production
Industrial enzymes are widely used inputs in the food, beverage, textile, detergent and feed sectors. In production processes run with bacterial, yeast and fungal cultures, yield depends on strain and medium properties. It also depends on managing oxygen transfer, feeding, pH, temperature, induction and harvest timing together.
Application-specific process requirements
Oxygen requirement
In aerobic processes the oxygen requirement is met by air flow and agitation and, where needed, by vessel pressure and oxygen enrichment; the limits of these variables are evaluated together.
Fed-batch operation
In fed-batch operation the feed profile is defined with regard to substrate inhibition, catabolite repression, overflow metabolism and oxygen demand. Profiles are entered on the control unit and applied with peristaltic pumps.
Harvest and primary separation interface
Transfer interfaces suited to harvest and primary separation equipment; the scope of subsequent purification steps is defined separately in the project.
Talamus models for this application
| Model | Scale | Working volume | Sterilisation | Delivery | Control structure |
|---|---|---|---|---|---|
| TLM Lab | Laboratory systems | Laboratory scale; vessel volume and number of parallel reactors per configuration | Autoclave | Benchtop | Shared loop and data structure |
| TLM120 | Pilot-scale systems | 20 L seed reactor + 100 L pilot reactor | Automated SIP | Skid-mounted | Shared phase, loop and data structure |
| TLM500 | Pilot-scale systems | 500 L working volume (600 L total) | Automated SIP | Skid-mounted | Shared phase, loop and data structure |
Technical consultation request
Information on the production organism, target enzyme, medium approach and target working volume is sufficient for an initial assessment.